SARS Coronavirus 3b Accessory Protein Modulates Transcriptional Activity of RUNX1b
Identifieur interne : 001304 ( Main/Exploration ); précédent : 001303; suivant : 001305SARS Coronavirus 3b Accessory Protein Modulates Transcriptional Activity of RUNX1b
Auteurs : Bhavna Varshney [Inde] ; Sudhakar Agnihotram [États-Unis] ; Yee-Joo Tan [Singapour] ; Ralph Baric [États-Unis] ; Sunil K. Lal [Inde]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Abstract
The causative agent of severe acute respiratory syndrome, SARS coronavirus (SARS-CoV) genome encodes several unique group specific accessory proteins with unknown functions. Among them, accessory protein 3b (also known as ORF4) was lately identified as one of the viral interferon antagonist. Recently our lab uncovered a new role for 3b in upregulation of AP-1 transcriptional activity and its downstream genes. Thus, we believe that 3b might play an important role in SARS-CoV pathogenesis and therefore is of considerable interest. The current study aims at identifying novel host cellular interactors of the 3b protein.
In this study, using yeast two-hybrid and co-immunoprecipitation techniques, we have identified a host transcription factor RUNX1b (Runt related transcription factor, isoform b) as a novel interacting partner for SARS-CoV 3b protein. Chromatin immunoprecipitaion (ChIP) and reporter gene assays in 3b expressing jurkat cells showed recruitment of 3b on the RUNX1 binding element that led to an increase in RUNX1b transactivation potential on the IL2 promoter. Kinase assay and pharmacological inhibitor treatment implied that 3b also affect RUNX1b transcriptional activity by regulating its ERK dependent phosphorylation levels. Additionally, mRNA levels of MIP-1α, a RUNX1b target gene upregulated in SARS-CoV infected monocyte-derived dendritic cells, were found to be elevated in 3b expressing U937 monocyte cells.
These results unveil a novel interaction of SARS-CoV 3b with the host factor, RUNX1b, and speculate its physiological relevance in upregulating cytokines and chemokine levels in state of SARS virus infection.
Url:
DOI: 10.1371/journal.pone.0029542
PubMed: 22253733
PubMed Central: 3257236
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000A11
- to stream Pmc, to step Curation: 000A11
- to stream Pmc, to step Checkpoint: 000734
- to stream Ncbi, to step Merge: 000301
- to stream Ncbi, to step Curation: 000301
- to stream Ncbi, to step Checkpoint: 000301
- to stream Main, to step Merge: 001306
- to stream Main, to step Curation: 001304
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS Coronavirus 3b Accessory Protein Modulates Transcriptional Activity of RUNX1b</title><author><name sortKey="Varshney, Bhavna" sort="Varshney, Bhavna" uniqKey="Varshney B" first="Bhavna" last="Varshney">Bhavna Varshney</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Virology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Agnihotram, Sudhakar" sort="Agnihotram, Sudhakar" uniqKey="Agnihotram S" first="Sudhakar" last="Agnihotram">Sudhakar Agnihotram</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="4"><nlm:aff id="aff3"><addr-line>Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Baric, Ralph" sort="Baric, Ralph" uniqKey="Baric R" first="Ralph" last="Baric">Ralph Baric</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K." last="Lal">Sunil K. Lal</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Virology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22253733</idno><idno type="pmc">3257236</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3257236</idno><idno type="RBID">PMC:3257236</idno><idno type="doi">10.1371/journal.pone.0029542</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000A11</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000A11</idno><idno type="wicri:Area/Pmc/Curation">000A11</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000A11</idno><idno type="wicri:Area/Pmc/Checkpoint">000734</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000734</idno><idno type="wicri:Area/Ncbi/Merge">000301</idno><idno type="wicri:Area/Ncbi/Curation">000301</idno><idno type="wicri:Area/Ncbi/Checkpoint">000301</idno><idno type="wicri:Area/Main/Merge">001306</idno><idno type="wicri:Area/Main/Curation">001304</idno><idno type="wicri:Area/Main/Exploration">001304</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">SARS Coronavirus 3b Accessory Protein Modulates Transcriptional Activity of RUNX1b</title><author><name sortKey="Varshney, Bhavna" sort="Varshney, Bhavna" uniqKey="Varshney B" first="Bhavna" last="Varshney">Bhavna Varshney</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Virology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author><author><name sortKey="Agnihotram, Sudhakar" sort="Agnihotram, Sudhakar" uniqKey="Agnihotram S" first="Sudhakar" last="Agnihotram">Sudhakar Agnihotram</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="4"><nlm:aff id="aff3"><addr-line>Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore</addr-line></nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore</wicri:regionArea><orgName type="university">Université nationale de Singapour</orgName></affiliation></author><author><name sortKey="Baric, Ralph" sort="Baric, Ralph" uniqKey="Baric R" first="Ralph" last="Baric">Ralph Baric</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, North Carolina</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K." last="Lal">Sunil K. Lal</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Virology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, India</addr-line></nlm:aff><country xml:lang="fr">Inde</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi</wicri:regionArea><wicri:noRegion>New Delhi</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The causative agent of severe acute respiratory syndrome, SARS coronavirus (SARS-CoV) genome encodes several unique group specific accessory proteins with unknown functions. Among them, accessory protein 3b (also known as ORF4) was lately identified as one of the viral interferon antagonist. Recently our lab uncovered a new role for 3b in upregulation of AP-1 transcriptional activity and its downstream genes. Thus, we believe that 3b might play an important role in SARS-CoV pathogenesis and therefore is of considerable interest. The current study aims at identifying novel host cellular interactors of the 3b protein.</p></sec><sec><title>Methodology/Principal Findings</title><p>In this study, using yeast two-hybrid and co-immunoprecipitation techniques, we have identified a host transcription factor RUNX1b (Runt related transcription factor, isoform b) as a novel interacting partner for SARS-CoV 3b protein. Chromatin immunoprecipitaion (ChIP) and reporter gene assays in 3b expressing jurkat cells showed recruitment of 3b on the RUNX1 binding element that led to an increase in RUNX1b transactivation potential on the IL2 promoter. Kinase assay and pharmacological inhibitor treatment implied that 3b also affect RUNX1b transcriptional activity by regulating its ERK dependent phosphorylation levels. Additionally, mRNA levels of MIP-1α, a RUNX1b target gene upregulated in SARS-CoV infected monocyte-derived dendritic cells, were found to be elevated in 3b expressing U937 monocyte cells.</p></sec><sec><title>Conclusions/Significance</title><p>These results unveil a novel interaction of SARS-CoV 3b with the host factor, RUNX1b, and speculate its physiological relevance in upregulating cytokines and chemokine levels in state of SARS virus infection.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name></author><author><name sortKey="Van Der Werf, S" uniqKey="Van Der Werf S">S van der Werf</name></author><author><name sortKey="Brodt, Hr" uniqKey="Brodt H">HR Brodt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ksiazek, Tg" uniqKey="Ksiazek T">TG Ksiazek</name></author><author><name sortKey="Erdman, D" uniqKey="Erdman D">D Erdman</name></author><author><name sortKey="Goldsmith, Cs" uniqKey="Goldsmith C">CS Goldsmith</name></author><author><name sortKey="Zaki, Sr" uniqKey="Zaki S">SR Zaki</name></author><author><name sortKey="Peret, T" uniqKey="Peret T">T Peret</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, Pa" uniqKey="Rota P">PA Rota</name></author><author><name sortKey="Oberste, Ms" uniqKey="Oberste M">MS Oberste</name></author><author><name sortKey="Monroe, Ss" uniqKey="Monroe S">SS Monroe</name></author><author><name sortKey="Nix, Wa" uniqKey="Nix W">WA Nix</name></author><author><name sortKey="Campagnoli, R" uniqKey="Campagnoli R">R Campagnoli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marra, Ma" uniqKey="Marra M">MA Marra</name></author><author><name sortKey="Jones, Sj" uniqKey="Jones S">SJ Jones</name></author><author><name sortKey="Astell, Cr" uniqKey="Astell C">CR Astell</name></author><author><name sortKey="Holt, Ra" uniqKey="Holt R">RA Holt</name></author><author><name sortKey="Brooks Wilson, A" uniqKey="Brooks Wilson A">A Brooks-Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, Jp" uniqKey="Guo J">JP Guo</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M Petric</name></author><author><name sortKey="Campbell, W" uniqKey="Campbell W">W Campbell</name></author><author><name sortKey="Mcgeer, Pl" uniqKey="Mcgeer P">PL McGeer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Ws" uniqKey="Chan W">WS Chan</name></author><author><name sortKey="Wu, C" uniqKey="Wu C">C Wu</name></author><author><name sortKey="Chow, Sc" uniqKey="Chow S">SC Chow</name></author><author><name sortKey="Cheung, T" uniqKey="Cheung T">T Cheung</name></author><author><name sortKey="To, Kf" uniqKey="To K">KF To</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yount, B" uniqKey="Yount B">B Yount</name></author><author><name sortKey="Roberts, Rs" uniqKey="Roberts R">RS Roberts</name></author><author><name sortKey="Sims, Ac" uniqKey="Sims A">AC Sims</name></author><author><name sortKey="Deming, D" uniqKey="Deming D">D Deming</name></author><author><name sortKey="Frieman, Mb" uniqKey="Frieman M">MB Frieman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kopecky Bromberg, Sa" uniqKey="Kopecky Bromberg S">SA Kopecky-Bromberg</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L Martinez-Sobrido</name></author><author><name sortKey="Frieman, M" uniqKey="Frieman M">M Frieman</name></author><author><name sortKey="Baric, Ra" uniqKey="Baric R">RA Baric</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Freundt, Ec" uniqKey="Freundt E">EC Freundt</name></author><author><name sortKey="Yu, L" uniqKey="Yu L">L Yu</name></author><author><name sortKey="Park, E" uniqKey="Park E">E Park</name></author><author><name sortKey="Lenardo, Mj" uniqKey="Lenardo M">MJ Lenardo</name></author><author><name sortKey="Xu, Xn" uniqKey="Xu X">XN Xu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, X" uniqKey="Yuan X">X Yuan</name></author><author><name sortKey="Shan, Y" uniqKey="Shan Y">Y Shan</name></author><author><name sortKey="Yao, Z" uniqKey="Yao Z">Z Yao</name></author><author><name sortKey="Li, J" uniqKey="Li J">J Li</name></author><author><name sortKey="Zhao, Z" uniqKey="Zhao Z">Z Zhao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, X" uniqKey="Yuan X">X Yuan</name></author><author><name sortKey="Yao, Z" uniqKey="Yao Z">Z Yao</name></author><author><name sortKey="Shan, Y" uniqKey="Shan Y">Y Shan</name></author><author><name sortKey="Chen, B" uniqKey="Chen B">B Chen</name></author><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Varshney, B" uniqKey="Varshney B">B Varshney</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Levanon, D" uniqKey="Levanon D">D Levanon</name></author><author><name sortKey="Groner, Y" uniqKey="Groner Y">Y Groner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ogawa, E" uniqKey="Ogawa E">E Ogawa</name></author><author><name sortKey="Inuzuka, M" uniqKey="Inuzuka M">M Inuzuka</name></author><author><name sortKey="Maruyama, M" uniqKey="Maruyama M">M Maruyama</name></author><author><name sortKey="Satake, M" uniqKey="Satake M">M Satake</name></author><author><name sortKey="Naito Fujimoto, M" uniqKey="Naito Fujimoto M">M Naito-Fujimoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyers, S" uniqKey="Meyers S">S Meyers</name></author><author><name sortKey="Downing, Jr" uniqKey="Downing J">JR Downing</name></author><author><name sortKey="Hiebert, Sw" uniqKey="Hiebert S">SW Hiebert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miyoshi, H" uniqKey="Miyoshi H">H Miyoshi</name></author><author><name sortKey="Ohira, M" uniqKey="Ohira M">M Ohira</name></author><author><name sortKey="Shimizu, K" uniqKey="Shimizu K">K Shimizu</name></author><author><name sortKey="Mitani, K" uniqKey="Mitani K">K Mitani</name></author><author><name sortKey="Hirai, H" uniqKey="Hirai H">H Hirai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ichikawa, M" uniqKey="Ichikawa M">M Ichikawa</name></author><author><name sortKey="Asai, T" uniqKey="Asai T">T Asai</name></author><author><name sortKey="Saito, T" uniqKey="Saito T">T Saito</name></author><author><name sortKey="Seo, S" uniqKey="Seo S">S Seo</name></author><author><name sortKey="Yamazaki, I" uniqKey="Yamazaki I">I Yamazaki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taniuchi, I" uniqKey="Taniuchi I">I Taniuchi</name></author><author><name sortKey="Osato, M" uniqKey="Osato M">M Osato</name></author><author><name sortKey="Egawa, T" uniqKey="Egawa T">T Egawa</name></author><author><name sortKey="Sunshine, Mj" uniqKey="Sunshine M">MJ Sunshine</name></author><author><name sortKey="Bae, Sc" uniqKey="Bae S">SC Bae</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ono, M" uniqKey="Ono M">M Ono</name></author><author><name sortKey="Yaguchi, H" uniqKey="Yaguchi H">H Yaguchi</name></author><author><name sortKey="Ohkura, N" uniqKey="Ohkura N">N Ohkura</name></author><author><name sortKey="Kitabayashi, I" uniqKey="Kitabayashi I">I Kitabayashi</name></author><author><name sortKey="Nagamura, Y" uniqKey="Nagamura Y">Y Nagamura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name></author><author><name sortKey="Huang, G" uniqKey="Huang G">G Huang</name></author><author><name sortKey="Zhao, X" uniqKey="Zhao X">X Zhao</name></author><author><name sortKey="Hatlen, Ma" uniqKey="Hatlen M">MA Hatlen</name></author><author><name sortKey="Vu, L" uniqKey="Vu L">L Vu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tanaka, T" uniqKey="Tanaka T">T Tanaka</name></author><author><name sortKey="Kurokawa, M" uniqKey="Kurokawa M">M Kurokawa</name></author><author><name sortKey="Ueki, K" uniqKey="Ueki K">K Ueki</name></author><author><name sortKey="Tanaka, K" uniqKey="Tanaka K">K Tanaka</name></author><author><name sortKey="Imai, Y" uniqKey="Imai Y">Y Imai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Menten, P" uniqKey="Menten P">P Menten</name></author><author><name sortKey="Wuyts, A" uniqKey="Wuyts A">A Wuyts</name></author><author><name sortKey="Van Damme, J" uniqKey="Van Damme J">J Van Damme</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bristow, Ca" uniqKey="Bristow C">CA Bristow</name></author><author><name sortKey="Shore, P" uniqKey="Shore P">P Shore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Law, Hk" uniqKey="Law H">HK Law</name></author><author><name sortKey="Cheung, Cy" uniqKey="Cheung C">CY Cheung</name></author><author><name sortKey="Ng, Hy" uniqKey="Ng H">HY Ng</name></author><author><name sortKey="Sia, Sf" uniqKey="Sia S">SF Sia</name></author><author><name sortKey="Chan, Yo" uniqKey="Chan Y">YO Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name></author><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Lokugamage, K" uniqKey="Lokugamage K">K Lokugamage</name></author><author><name sortKey="Kamitani, W" uniqKey="Kamitani W">W Kamitani</name></author><author><name sortKey="Ikegami, T" uniqKey="Ikegami T">T Ikegami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Siu, Kl" uniqKey="Siu K">KL Siu</name></author><author><name sortKey="Kok, Kh" uniqKey="Kok K">KH Kok</name></author><author><name sortKey="Ng, Mh" uniqKey="Ng M">MH Ng</name></author><author><name sortKey="Poon, Vk" uniqKey="Poon V">VK Poon</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Golub, Tr" uniqKey="Golub T">TR Golub</name></author><author><name sortKey="Barker, Gf" uniqKey="Barker G">GF Barker</name></author><author><name sortKey="Bohlander, Sk" uniqKey="Bohlander S">SK Bohlander</name></author><author><name sortKey="Hiebert, Sw" uniqKey="Hiebert S">SW Hiebert</name></author><author><name sortKey="Ward, Dc" uniqKey="Ward D">DC Ward</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lutterbach, B" uniqKey="Lutterbach B">B Lutterbach</name></author><author><name sortKey="Westendorf, Jj" uniqKey="Westendorf J">JJ Westendorf</name></author><author><name sortKey="Linggi, B" uniqKey="Linggi B">B Linggi</name></author><author><name sortKey="Isaac, S" uniqKey="Isaac S">S Isaac</name></author><author><name sortKey="Seto, E" uniqKey="Seto E">E Seto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mikhail, Fm" uniqKey="Mikhail F">FM Mikhail</name></author><author><name sortKey="Serry, Ka" uniqKey="Serry K">KA Serry</name></author><author><name sortKey="Hatem, N" uniqKey="Hatem N">N Hatem</name></author><author><name sortKey="Mourad, Zi" uniqKey="Mourad Z">ZI Mourad</name></author><author><name sortKey="Farawela, Hm" uniqKey="Farawela H">HM Farawela</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Starkova, J" uniqKey="Starkova J">J Starkova</name></author><author><name sortKey="Madzo, J" uniqKey="Madzo J">J Madzo</name></author><author><name sortKey="Cario, G" uniqKey="Cario G">G Cario</name></author><author><name sortKey="Kalina, T" uniqKey="Kalina T">T Kalina</name></author><author><name sortKey="Ford, A" uniqKey="Ford A">A Ford</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taylor, Ds" uniqKey="Taylor D">DS Taylor</name></author><author><name sortKey="Laubach, Jp" uniqKey="Laubach J">JP Laubach</name></author><author><name sortKey="Nathan, Dg" uniqKey="Nathan D">DG Nathan</name></author><author><name sortKey="Mathey Prevot, B" uniqKey="Mathey Prevot B">B Mathey-Prevot</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Uchida, H" uniqKey="Uchida H">H Uchida</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Nimer, Sd" uniqKey="Nimer S">SD Nimer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cockerill, Pn" uniqKey="Cockerill P">PN Cockerill</name></author><author><name sortKey="Osborne, Cs" uniqKey="Osborne C">CS Osborne</name></author><author><name sortKey="Bert, Ag" uniqKey="Bert A">AG Bert</name></author><author><name sortKey="Grotto, Rj" uniqKey="Grotto R">RJ Grotto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, De" uniqKey="Zhang D">DE Zhang</name></author><author><name sortKey="Fujioka, K" uniqKey="Fujioka K">K Fujioka</name></author><author><name sortKey="Hetherington, Cj" uniqKey="Hetherington C">CJ Hetherington</name></author><author><name sortKey="Shapiro, Lh" uniqKey="Shapiro L">LH Shapiro</name></author><author><name sortKey="Chen, Hm" uniqKey="Chen H">HM Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nuchprayoon, I" uniqKey="Nuchprayoon I">I Nuchprayoon</name></author><author><name sortKey="Meyers, S" uniqKey="Meyers S">S Meyers</name></author><author><name sortKey="Scott, Lm" uniqKey="Scott L">LM Scott</name></author><author><name sortKey="Suzow, J" uniqKey="Suzow J">J Suzow</name></author><author><name sortKey="Hiebert, S" uniqKey="Hiebert S">S Hiebert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, De" uniqKey="Zhang D">DE Zhang</name></author><author><name sortKey="Hetherington, Cj" uniqKey="Hetherington C">CJ Hetherington</name></author><author><name sortKey="Meyers, S" uniqKey="Meyers S">S Meyers</name></author><author><name sortKey="Rhoades, Kl" uniqKey="Rhoades K">KL Rhoades</name></author><author><name sortKey="Larson, Cj" uniqKey="Larson C">CJ Larson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wotton, D" uniqKey="Wotton D">D Wotton</name></author><author><name sortKey="Ghysdael, J" uniqKey="Ghysdael J">J Ghysdael</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author><author><name sortKey="Speck, Na" uniqKey="Speck N">NA Speck</name></author><author><name sortKey="Owen, Mj" uniqKey="Owen M">MJ Owen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Elagib, Ke" uniqKey="Elagib K">KE Elagib</name></author><author><name sortKey="Racke, Fk" uniqKey="Racke F">FK Racke</name></author><author><name sortKey="Mogass, M" uniqKey="Mogass M">M Mogass</name></author><author><name sortKey="Khetawat, R" uniqKey="Khetawat R">R Khetawat</name></author><author><name sortKey="Delehanty, Ll" uniqKey="Delehanty L">LL Delehanty</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kitabayashi, I" uniqKey="Kitabayashi I">I Kitabayashi</name></author><author><name sortKey="Yokoyama, A" uniqKey="Yokoyama A">A Yokoyama</name></author><author><name sortKey="Shimizu, K" uniqKey="Shimizu K">K Shimizu</name></author><author><name sortKey="Ohki, M" uniqKey="Ohki M">M Ohki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kitabayashi, I" uniqKey="Kitabayashi I">I Kitabayashi</name></author><author><name sortKey="Aikawa, Y" uniqKey="Aikawa Y">Y Aikawa</name></author><author><name sortKey="Nguyen, La" uniqKey="Nguyen L">LA Nguyen</name></author><author><name sortKey="Yokoyama, A" uniqKey="Yokoyama A">A Yokoyama</name></author><author><name sortKey="Ohki, M" uniqKey="Ohki M">M Ohki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Javed, A" uniqKey="Javed A">A Javed</name></author><author><name sortKey="Guo, B" uniqKey="Guo B">B Guo</name></author><author><name sortKey="Hiebert, S" uniqKey="Hiebert S">S Hiebert</name></author><author><name sortKey="Choi, Jy" uniqKey="Choi J">JY Choi</name></author><author><name sortKey="Green, J" uniqKey="Green J">J Green</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imai, Y" uniqKey="Imai Y">Y Imai</name></author><author><name sortKey="Kurokawa, M" uniqKey="Kurokawa M">M Kurokawa</name></author><author><name sortKey="Tanaka, K" uniqKey="Tanaka K">K Tanaka</name></author><author><name sortKey="Friedman, Ad" uniqKey="Friedman A">AD Friedman</name></author><author><name sortKey="Ogawa, S" uniqKey="Ogawa S">S Ogawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Levanon, D" uniqKey="Levanon D">D Levanon</name></author><author><name sortKey="Goldstein, Re" uniqKey="Goldstein R">RE Goldstein</name></author><author><name sortKey="Bernstein, Y" uniqKey="Bernstein Y">Y Bernstein</name></author><author><name sortKey="Tang, H" uniqKey="Tang H">H Tang</name></author><author><name sortKey="Goldenberg, D" uniqKey="Goldenberg D">D Goldenberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaiman, Al" uniqKey="Zaiman A">AL Zaiman</name></author><author><name sortKey="Lewis, Af" uniqKey="Lewis A">AF Lewis</name></author><author><name sortKey="Crute, Be" uniqKey="Crute B">BE Crute</name></author><author><name sortKey="Speck, Na" uniqKey="Speck N">NA Speck</name></author><author><name sortKey="Lenz, J" uniqKey="Lenz J">J Lenz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaiman, Al" uniqKey="Zaiman A">AL Zaiman</name></author><author><name sortKey="Lenz, J" uniqKey="Lenz J">J Lenz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sutton, Ka" uniqKey="Sutton K">KA Sutton</name></author><author><name sortKey="Lin, Ct" uniqKey="Lin C">CT Lin</name></author><author><name sortKey="Harkiss, Gd" uniqKey="Harkiss G">GD Harkiss</name></author><author><name sortKey="Mcconnell, I" uniqKey="Mcconnell I">I McConnell</name></author><author><name sortKey="Sargan, Dr" uniqKey="Sargan D">DR Sargan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Speck, Na" uniqKey="Speck N">NA Speck</name></author><author><name sortKey="Terryl, S" uniqKey="Terryl S">S Terryl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schmidt, Hm" uniqKey="Schmidt H">HM Schmidt</name></author><author><name sortKey="Steger, G" uniqKey="Steger G">G Steger</name></author><author><name sortKey="Pfister, H" uniqKey="Pfister H">H Pfister</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boeckle, S" uniqKey="Boeckle S">S Boeckle</name></author><author><name sortKey="Pfister, H" uniqKey="Pfister H">H Pfister</name></author><author><name sortKey="Steger, G" uniqKey="Steger G">G Steger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brady, G" uniqKey="Brady G">G Brady</name></author><author><name sortKey="Whiteman, Hj" uniqKey="Whiteman H">HJ Whiteman</name></author><author><name sortKey="Spender, Lc" uniqKey="Spender L">LC Spender</name></author><author><name sortKey="Farrell, Pj" uniqKey="Farrell P">PJ Farrell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Slobedman, B" uniqKey="Slobedman B">B Slobedman</name></author><author><name sortKey="Stern, Jl" uniqKey="Stern J">JL Stern</name></author><author><name sortKey="Cunningham, Al" uniqKey="Cunningham A">AL Cunningham</name></author><author><name sortKey="Abendroth, A" uniqKey="Abendroth A">A Abendroth</name></author><author><name sortKey="Abate, Da" uniqKey="Abate D">DA Abate</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marshall, Lj" uniqKey="Marshall L">LJ Marshall</name></author><author><name sortKey="Moore, Ac" uniqKey="Moore A">AC Moore</name></author><author><name sortKey="Ohki, M" uniqKey="Ohki M">M Ohki</name></author><author><name sortKey="Kitabayashi, I" uniqKey="Kitabayashi I">I Kitabayashi</name></author><author><name sortKey="Patterson, D" uniqKey="Patterson D">D Patterson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tang, Bs" uniqKey="Tang B">BS Tang</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamelin, V" uniqKey="Hamelin V">V Hamelin</name></author><author><name sortKey="Letourneux, C" uniqKey="Letourneux C">C Letourneux</name></author><author><name sortKey="Romeo, Ph" uniqKey="Romeo P">PH Romeo</name></author><author><name sortKey="Porteu, F" uniqKey="Porteu F">F Porteu</name></author><author><name sortKey="Gaudry, M" uniqKey="Gaudry M">M Gaudry</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Lau, Yf" uniqKey="Lau Y">YF Lau</name></author><author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name></author><author><name sortKey="Paddock, Cd" uniqKey="Paddock C">CD Paddock</name></author><author><name sortKey="Bartlett, Jh" uniqKey="Bartlett J">JH Bartlett</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Khan, S" uniqKey="Khan S">S Khan</name></author><author><name sortKey="Fielding, Bc" uniqKey="Fielding B">BC Fielding</name></author><author><name sortKey="Tan, Th" uniqKey="Tan T">TH Tan</name></author><author><name sortKey="Chou, Cf" uniqKey="Chou C">CF Chou</name></author><author><name sortKey="Shen, S" uniqKey="Shen S">S Shen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tyagi, S" uniqKey="Tyagi S">S Tyagi</name></author><author><name sortKey="Surjit, M" uniqKey="Surjit M">M Surjit</name></author><author><name sortKey="Roy, Ak" uniqKey="Roy A">AK Roy</name></author><author><name sortKey="Jameel, S" uniqKey="Jameel S">S Jameel</name></author><author><name sortKey="Lal, Sk" uniqKey="Lal S">SK Lal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Korkaya, H" uniqKey="Korkaya H">H Korkaya</name></author><author><name sortKey="Jameel, S" uniqKey="Jameel S">S Jameel</name></author><author><name sortKey="Gupta, D" uniqKey="Gupta D">D Gupta</name></author><author><name sortKey="Tyagi, S" uniqKey="Tyagi S">S Tyagi</name></author><author><name sortKey="Kumar, R" uniqKey="Kumar R">R Kumar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Surjit, M" uniqKey="Surjit M">M Surjit</name></author><author><name sortKey="Liu, B" uniqKey="Liu B">B Liu</name></author><author><name sortKey="Chow, Vt" uniqKey="Chow V">VT Chow</name></author><author><name sortKey="Lal, Sk" uniqKey="Lal S">SK Lal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luo, Rx" uniqKey="Luo R">RX Luo</name></author><author><name sortKey="Postigo, Aa" uniqKey="Postigo A">AA Postigo</name></author><author><name sortKey="Dean, Dc" uniqKey="Dean D">DC Dean</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Inde</li><li>Singapour</li><li>États-Unis</li></country><region><li>Caroline du Nord</li></region><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Inde"><noRegion><name sortKey="Varshney, Bhavna" sort="Varshney, Bhavna" uniqKey="Varshney B" first="Bhavna" last="Varshney">Bhavna Varshney</name></noRegion><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K." last="Lal">Sunil K. Lal</name></country><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Agnihotram, Sudhakar" sort="Agnihotram, Sudhakar" uniqKey="Agnihotram S" first="Sudhakar" last="Agnihotram">Sudhakar Agnihotram</name></region><name sortKey="Baric, Ralph" sort="Baric, Ralph" uniqKey="Baric R" first="Ralph" last="Baric">Ralph Baric</name></country><country name="Singapour"><noRegion><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001304 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001304 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:3257236
|texte= SARS Coronavirus 3b Accessory Protein Modulates Transcriptional Activity of RUNX1b
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22253733" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a CovidV2
| This area was generated with Dilib version V0.6.33. |  |